膨胀床吸附层析技术基础性能研究进展

[an error occurred while processing this directive]

化学工业与工程

化学工业与工程

2004, Vol. 21

Issue (4) :254-258 DOI:

论文

最新目录 | 下期目录 | 过刊浏览 | 高级检索

<< | >>

膨胀床吸附层析技术基础性能研究进展

关红欣,胡洪波,张雪洪,唐涌濂

上海交通大学生命科学技术学院,上海交通大学生命科学技术学院,上海交通大学生命科学技术学院,上海交通大学生命科学技术学院上海200240 ,上海200240 ,上海200240 ,上海200240

Progress on Basic Features of Expanded Bed Adsorption Chromatography

GUAN Hong-xin, HU Hong-bo, ZHANG Xue-hong, TANG Yong-lian (Life Institute of Science and Technology, Shang Hai Jiao Tong University, Shanghai 200240, China)

(Life Institute of Science and Technology, Shang Hai Jiao Tong University, Shanghai 200240, China

摘要	参考文献	相关文章

Download: PDF (0KB) HTML () Export: BibTeX or EndNote (RIS) Supporting Info

摘要膨胀床吸附层析技术兼有流化床和填充床层析的优点,不需预先除去料液中的颗粒而可以直接从料液中吸附目标产物。但该技术还不成熟,操作还有很大的不确定性,为了解其基础性能,掌握其膨胀和吸附机制,本文从膨胀床流体力学性能、吸附性能、吸附剂与细胞和细胞碎片之间的相互作用等方面,对近年来国内外膨胀床基础性能的研究进行了综述。

	Service

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	Email Alert
	RSS
	作者相关文章
	关红欣
	胡洪波
	张雪洪
	唐涌濂

关键词：膨胀床层析吸附流体力学性能

Abstract： With merits of both fluidized bed and packed bed,the technology of expanded bed adsorption chromatography can capture product directly from the crude feed without removal of solid particles previously.But the technology is not well developed and operations are instable.In order to probe into the mechanism of expansion and adsorption,researches on basic characteristics of expanded bed,such as hydrodynamics,adsorption,interaction of cell and adsorption etc,in recent years are reviewed.

Keywords：

Received 2004-07-15; published 2004-07-15

引用本文:

关红欣,胡洪波,张雪洪,唐涌濂.膨胀床吸附层析技术基础性能研究进展[J]. 化学工业与工程, 2004,21(4): 254-258

GUAN Hong-xin, HU Hong-bo, ZHANG Xue-hong, TANG Yong-lian .Progress on Basic Features of Expanded Bed Adsorption Chromatography[J]. Chemcial Industry and Engineering, 2004,21(4): 254-258

[1]	CHASEHA,DREAGERNM.Affinitypurificationofproteinsusingexpandedbeds[J].Chromatogr.1992,597:129-145
[2]	胡洪波,姚善泾,朱自强.膨胀床吸附层析技术及其在生化分离中的应用[J].化学工程,1999,27(1):37-41.
[3]	CHANGYK,CHASEHA.Developmentofoperatingconditionsforproteinpurificationusingexpandedbedtechniques:Theeffectsofthedegreeofbedexpandedonadsorptionperformance[J].BiotechnolBioeng,1996,49:204.
[4]	TH MMESJ,BADERA,HALFARM,etal.IsolationofmonoclonalantibodiesfromcellcontaininghybridomabrothusingaproteinAcoatedadsorbentinexpandedbed[J].Chromatogr.1996,752:111-
[5]	RICHARDSONJF,ZAKIWN.Sedimentationandfluidization:PartⅠ[J].TransInstChemEng,1954,32:35-53.
[6]	DASARIG,PRINCEI,HEARNMTW.Highperformanceliquidchromatographyofaminoacids,peptidesandproteinsCXXIV.Physicalcharacterizationoffluidized-bedbehaviourofchromatographicpakingmaterials[J]..Chromatogr.1993,631:115-124
[7]	TH MMESJ,HALFARM,LENS,etal.Purificationofmonoclonalantibodiesfromwholehybridomafermentationbrothbyfluidizedbedadsorption[J].BiotechnolBioeng,1995,45(3):205-211.
[8]	KARAUA,BENKENC,TH MMESJ,etal.Theinfluenceofparticlesizedistributionandoperatingandconditionsontheadsorptionperformanceinfluidizedbeds[J].BiotechnolBioeng,1997,55:54-64.
[9]	CARLOSCR,RICHARDSONJF.Solidmovementinliquidfluidizedbeds-Ⅱ.Measurementsofaxialmixingcoefficients[J].ChemEngSci,1968,23:825-831.
[10]	胡洪波,林东强,叶子坚,等.膨胀床的膨胀特征和流体混合性能[J].高校化学工程学报,2000,14(2):139-144.
[11]	JOHANSSONBU,WNUKOWSKIP.Hydrodynamicstabilityofliquidfluidizedbedforsmallparticles:Anexperimentalstudy[R].USAFlorida:AIChEAnnualMeeting,1992.
[12]	TH MMESJ,WEIHERM,KARAUA,etal.Hydrodynamicsandperformanceinfluidizedbedadsorption[J].BiotechnolBioeng,1995,48:376-374.
[13]	BRUCELJ,CHASEHA.Evaluationoftheeffectofin-bedsamplingonexpandedbedadsorption[J].Bioseparation.1999,8:77-83
[14]	BRUCELJ,CHASEHA.Hydrodynamicsandadsorptionbehaviourwithinanexpandedbedadsorptioncolumnstudiedusingin-bedsampling[J].ChemicalEngineeringScience,2001,56:3149-3162.
[15]	佟晓东,杨　诤,孙　彦.第十届全国生物化工学术会议论文集[C].北京:中国化工学会生物化工专业委员会,2002.
[16]	HJORTHR,KAMPES,CARLSSONM.Analysisofsomeoperatingparametersofnoveladsorbentsforrecoveryofproteinsinexpandedbeds[J].Bioseparation,1995,5:217-223.
[17]	HALLKR,EAGLETONLC,AERIVOSA,etal.Poreandsolid-diffusionkineticsinfixedbedadsorptionunderconstant-patternconditions[J].I＆ECFund,1996,5:212-223.
[18]	刘　春,夏素兰,朱家骅.第七届全国生物化工会议论文集[C].南京:中国化工学会生物化工专业委员会,1998.
[19]	胡洪波,朱自强,姚善泾.扩张床吸附过程研究———安通溶栓酶的分离与纯化[D].杭州:浙江大学,1999.
[20]	BRUCELJ,CHASEHA.Thecombineduseofin-bedmonitoringandanadsorptionmodeltoanticipatebreakthroughduringexpandedbedadsorption[J].ChemicalEngineeringScience,2002,57:3085-3093.
[21]	PAMELAR,WRIGHT,BENJAMINJ.Modelingmasstransferandhydrodynamicsinfluidized-bedadsorptionofproteins[J].BioengineeringFoodandNaturalProducts,2001,2:474-488.
[22]	ERICKSONJC,FINCHJD,GREENEDC.Directcaptureofrecombinantproteinsfromanimalcellculturemediausingafluidizedbedadsorber[M].Oxford,UK:EDS,1994.
[23]	JANFEUSER,JOACHIMWALTER,MARIA-REGINAKULA,etal.Cell adsorbentinteractionsinexpandedbedadsorptionofproteins[J].Bioseparation.1999,8:99-109
[24]	FEUSERJ,HALFARM,L TKEMEYERD,etal.Interactionofmammaliancellculturebrothwithadsorbentsinexpandedbedadsorptionofmonoclonalantibodies[J].ProcessBiochemistry,1999,34:159-165.
[25]	FEN NDEZ-LAHOREHM,GEILENKIRCHENS,BOLDTK,etal.Theinfluenceofcelladsorbentinteractionsonproteinadsorptioninexpandedbeds[J].JournalofChromatography,2000,873:195-208.
[26]	LINDong-qiang,HECTORMARCELO,FEN NDEZ-LAHOREHM,etal.Minimisingbiomass adsorbentinteractionsinexpandedbedadsorptionprocesses:Amethodologicaldesignapproach[J].Bioseparation.2001,10:7-19
[27]	SIDDHARTHAGHOSE,HOWARDCHASE.ExpandedbedchromatographyofproteinsinsmalldiametercolumnsI.Scaledownandvalidation[J]..Bioseparation.2000,9:21-28
[28]	DASARIG,PRINCEI,HEARMTW.HighperformanceliquidchromatographyofaminoacidspeptidesandproteinsCXXIVPhysicalcharacterisationoffluidisedbedbehaviourofchromatographicpackingmaterials[J]..Chromatogr.1993,631:115-124