Preparation of Tanshinones from Salvia Miltiorrhiza Bunge by HSCCC
QU Yi~1,YANG Yue-wu~2,GUO Zhi-xin~2,ZHU Guo-guang~2(1.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;2.Tasly Group,Tianjin 300402,China)
(1.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;2.Tasly Group,Tianjin 300402,China
Abstract��
Separation and preparation of tanshinones from Danshen(Salvia miltiorrhiza Bunge) crude ethanol extract were studied by high-speed counter-current chromatography(HSCCC).The solvent system of petroleum ether-ethyl acetate-methanol-water(8��6��7��3) was selected and the optimum running parameters for apparatus was obtained through orthogonal experiment.Five tanshinone were separated and identified rapidly.Measured by HPLC,the purity of tanshinone ��A,cryptotanshinone,tanshinone ��,dihydrotanshinone �� were all over 98%.HSCCC is simple,economical,quick and with good repeatability for preparation of Danshen active components.
QU Yi~1,YANG Yue-wu~2,GUO Zhi-xin~2,ZHU Guo-guang~2.Preparation of Tanshinones from Salvia Miltiorrhiza Bunge by HSCCC[J]. Chemcial Industry and Engineering, 2007,24(1): 48-51
2007, Vol. 24 
