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ժҪ ��ø�ЧҺ��ɫ��-��⼼��(HPLC-UV)��߾�Ҷ��ȡ��з��,��˵ķ��-ˮ(��Ϊ32��68)��ϵ�ȶ�ϴ��,��0.7 mL/min,��Ⲩ��203 nmʱ,��߾�Ҷ��ȡ��и��ֵõ��˽Ϻõķ��롣��鷽��кܸߵľ��,�ȶ��Ժ��ԡ�Ϊ�˽�һ��о��߾�Ҷ��ȡ��ָ��ͼ��,��10��߾�Ҷ��ȡ��Ʒ��ͬ��ɫ��½��з��,��߾�Ҷ��ȡ��ȷ��14��з塱,��Ϊ��ָ��ͼ�ס��,��ø�ЧҺ��ɫ��-��ü��(HPLC-MS),��ղ��߰�ı�׼Ʒ,��Ѿ�ȷ��14��з塱��ԡ��߾�Ҷ��ȡ��ָ��ͼ�׵Ľ��ڽ��м�⡣

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�ؼ���� ߾�Ҷ��ȡ�� ЧҺ��ɫ�� ָ��ͼ��

Abstract�� At present,the construction of chromatographic fingerprints plays a significant role in the quality control of traditional medicines.In this paper,high performance liquid chromatography-ultraviolet absorption detector(HPLC-UV) profiles of saponins in panax notoginseng leaves were established.The analysis was carried out on a reversed-phase column(150 mm��4.6 mm,I.D.C_(18),5 ��m,) from Agilent maintained at 40 ��.Separation was performed by elution using acetonitrile and water at a volume ratio of 32��68.The flow rate was 0.7 mL/min.The detection wavelength was set at 203 nm.Under the above condition,peaks were well separated.To develop the representative fingerprint of saponins in panax notoginseng leaves,10 batches of samples were analyzed under the same HPLC conditions.The results showed that 10 batches of saponins in panax notoginseng leaves have 14 common peaks as their fingerprint.Besides,these common peaks were all characterized by using HPLC-MS technique and comparison with the reference standards.The fingerprint established will help to control the quality of saponins in panax notoginseng leaves.

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Received 2007-05-15; published 2007-05-15

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�׺��;��;��;.��߾�Ҷ��ȡ��ָ��ͼ�׵Ľ��[J]. ��ѧ��ҵ�빤��, 2007,24(3): 211-214

KONG Hai-ning,HAN Jin-yu,HUANG Xin.Construction of Chromatographic Fingerprint of Saponins in Panax Notoginseng Leaves[J]. Chemcial Industry and Engineering, 2007,24(3): 211-214