Investigation on L-Arginine Precipitated by Silver Nitrate
LIU Feng-ying,WENG Lian-jin,YANG Xin,GAN Lin-huo(Department of Chemical and Pharmaceutical Engineering,Huaqiao University,Quanzhou 362021,Fujian Province,China)
(Department of Chemical and Pharmaceutical Engineering,Huaqiao University,Quanzhou 362021,Fujian Province,China
Abstract：
L-arginine is precipitated when hot barium hydroxide is added to a solution containing arginine together with silver nitrate.Effects of temperature,pH,mole ratio of silver nitrate to L-arginine,initial L-arginine concentration,NH~+_4 and Na~+ concentration and mass ratio of L-lysine to L-arginine on the yield of L-arginine precipitation were investigated.Results show that,temperature has little effect on the precipitation yield,the maximum precipitation yield is reached at pH higher than 11.0.The mole ratio of silver nitrate to L-arginine should be higher than 2.0 and the initial Larginine concentration should be more than 10.0 g/L to keep over 90.0% precipitation yield.The precipitation yield hardly changes with the increase of Na~+ concentration but decreases sharply with the increase of NH~+_4 concentration and the increase of mass ratio of L-lysine to L-arginine.At last,we purified L-Arg from the precipitation using of 2.0 mol/L HCl,L-Arg of the solution was detected by HPCE.
LIU Feng-ying,WENG Lian-jin,YANG Xin,GAN Lin-huo.Investigation on L-Arginine Precipitated by Silver Nitrate[J]. Chemcial Industry and Engineering, 2009,26(2): 119-123
[1]
李良铸,李明晔.最新生化药物制备技术[M].北京:中国医药科技出版社,2000.
[2]
GREENSTEIN J P,WINITZ M.Chemistry of the AminoAcids[M].New York:Wiley,1961.
[3]
VICKERY H B,LEAVENWORTH C S.On the separationof histidine and arginineⅡ,the separation of the silvercompounds at pH 7.0[J].J Biol Chem,1927,72:403-413.
[4]
BLOCK R J.The determination of the basic amino acids insmall quantities of proteins by the silver precipitation method[J].J Biol Chem,1934,106:457-462.
2009, Vol. 26 
