碳点-金纳米簇结合的双发射荧光探针的制备及传感应用

摘要先以柠檬酸为原料采用水热合成法合成了碳点（CD），再以乙酰化含硫超支化聚酰胺-胺[HPA（S）-Ac]为模板和还原剂还原氯金酸（HAuCl₄）制备了金纳米簇（AuNCs），碳点和金纳米簇溶液混合后得到双发射荧光探针（CD-AuNCs），透射电镜结果表明CD和AuNCs粒径均接近2 nm，在CD-AuNCs中分散均匀。考察pH值对CD-AuNCs荧光性能的影响时发现CD-AuNCs的荧光在pH=6达到最强，且在此pH值下CD-AuNCs对四环素类药物（TCs）的响应灵敏，能够实现对TCs的传感。CD-AuNCs探针不仅选择性好，而且和单一CD探针相比具有线性范围更宽、灵敏度更高的优点。

	Service

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	Email Alert
	RSS
	作者相关文章
	张晓音
	余莉萍

关键词：碳点；金纳米簇；四环素

Abstract： Carbon dots (CD) were synthesized from citric acid using a hydrothermal method, and gold nanoclusters (AuNCs) were prepared from chloroauric acid (HAuCl₄) with sulfur-containing hyperbranched polyamide-amines as template and reducing agent. Then, the double-emitting fluorescent probes (CD-AuNCs) were obtained by mixing carbon dots and gold nanoclusters. Transmission electron microscopy images showed that the particle diameters of CD both were about 2 nm and dispersed uniformly. The effect of pH on the fluorescence performance of CD-AuNCs was investigated. It was found that the fluorescence of CD-AuNCs reached the strongest at pH=6, and CD-AuNCs responsed sensitively to tetracyclines (TCs) at this pH. The CD-AuNCs probe is not only highly selective, but also has the advantages of a wider linear range and a higher sensitivity than the single CD probe.

Keywords： carbon dots； gold nanoclusters； tetracyclines

Received 2019-01-26;

Corresponding Authors: 余莉萍,E-mail:lipingyu@tju.edu.cn。 Email: lipingyu@tju.edu.cn

About author: 张晓音(1993-),女,硕士研究生,主要研究方向纳米荧光探针的制备及传感应用。

引用本文:

张晓音, 余莉萍.碳点-金纳米簇结合的双发射荧光探针的制备及传感应用[J]. 化学工业与工程, 2020,37(2): 54-59

Zhang Xiaoyin, Yu Liping.Preparation and Application of Dual-Emission Fluorescent Probe Based on Carbon Dots Coupled with Gold Nanoclusters[J]. Chemcial Industry and Engineering, 2020,37(2): 54-59

[1] Yao J, Yang M, Liu Y, et al. Fluorescent CdS quantum dots:Synthesis, characterization, mechanism and interaction with gold nanoparticles[J]. Journal of Nanoscience and Nanotechnology, 2015, 15(5):3720-3727
[2] An X, Zhuo S, Zhang P, et al. Carbon dots based turn-on fluorescent probes for oxytetracycline hydrochloride sensing[J]. RSC Advances, 2015, 5(26):19853-19858
[3] Yang K, Wang S, Wang Y, et al. Dual-Channel probe of carbon dots cooperating with gold nanoclusters employed for assaying multiple targets[J]. Biosensors and Bioelectronics, 2017, 91:566-573
[4] Joseph J, Anappara A A. Microwave-Assisted hydrothermal synthesis of UV-emitting carbon dots from tannic acid[J]. New Journal of Chemistry, 2016, 40(9):8110-8117
[5] Yan F, Zou Y, Wang M, et al. Highly photoluminescent carbon dots-based fluorescent chemosensors for sensitive and selective detection of mercury ions and application of imaging in living cells[J]. Sensors and Actuators B:Chemical, 2014, 192:488-495
[6] Qu K, Wang J, Ren J, et al. Carbon dots prepared by hydrothermal treatment of dopamine as an effective fluorescent sensing platform for the label-free detection of iron(III) ions and dopamine[J]. Chemistry-A European Journal, 2013, 19(22):7243-7249
[7] Shi Y, Pan Y, Zhang H, et al. A dual-mode nanosensor based on carbon quantum dots and gold nanoparticles for discriminative detection of glutathione in human plasma[J]. Biosensors and Bioelectronics, 2014, 56:39-45
[8] Kaur N, Aditya R N, Singh A, et al. Biomedical applications for gold nanoclusters:Recent developments and future perspectives[J]. Nanoscale Research Letters, 2018, 13:1-12
[9] Wang Y, Mao L, Liu W, et al. A ratiometric fluorometric and colorimetric probe for the β-thalassemia drug deferiprone based on the use of gold nanoclusters and carbon dots[J]. Microchimica Acta, 2018, 185(9):1-9
[10] Yang X, Zhu S, Dou Y, et al. Novel and remarkable enhanced-fluorescence system based on gold nanoclusters for detection of tetracycline[J]. Talanta, 2014, 122:36-42
[11] Yan Y, Yu H, Zhang K, et al. Dual-Emissive nanohybrid of carbon dots and gold nanoclusters for sensitive determination of mercuric ions[J]. Nano Research, 2016, 9(7):2088-2096
[12] Huang D, Niu C, Ruan M, et al. Highly sensitive strategy for Hg²⁺ detection in environmental water samples using long lifetime fluorescence quantum dots and gold nanoparticles[J]. Environmental Science & Technology, 2013, 47(9):4392-4398
[13] Zhu H, Yu T, Xu H, et al. Fluorescent nanohybrid of gold nanoclusters and quantum dots for visual determination of lead ions[J]. ACS Applied Materials & Interfaces, 2014, 6(23):21461-21467
[14] Niu W, Shan D, Zhu R, et al. Dumbbell-Shaped carbon quantum dots/AuNCs nanohybrid as an efficient ratiometric fluorescent probe for sensing cadmium (II) ions and L-ascorbic acid[J]. Carbon, 2016, 96:1034-1042
[15] He Y, Pan C, Cao H, et al. Highly sensitive and selective dual-emission ratiometric fluorescence detection of dopamine based on carbon dots-gold nanoclusters hybrid[J]. Sensors and Actuators B:Chemical, 2018, 265:371-377
[16] Qu F, Xu X, You J. A new dual-emission fluorescence sensor based on carbon nanodots and gold nanoclusters for the detection of melamine[J]. New Journal of Chemistry, 2017, 41(17):9438-9443
[17] 路晨, 余莉萍. 超支化聚乙烯亚胺改性荧光碳点材料的制备和表征[J]. 化学工业与工程, 2019, 36(3):16-21 Lu Chen, Yu Liping. Preparation and characterization of fluorescent carbon dots modified by hyperbranched polyethyleneimine[J]. Chemical Industry and Engineering, 2019, 36(3):16-21(in Chinese)
[18] Zhang T, Zhan C, Chen Y, et al. Disulfide-Functionalized hyperbranched poly(amidoamine) derivatives as both reductant and stabilizer for the synthesis of fluorescent gold nanoclusters[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2017, 535:206-214
[19] 韩荣成, 喻敏, 沙印林. 溶液环境中CdSeS量子点与金纳米颗粒相互作用[J]. 物理化学学报, 2011, 27(1):255-261 Han Rongcheng, Yu Min, Sha Yinlin. Interaction between CdSeS quantum dots and gold nanoparticles in solution[J]. Acta Physico-Chimica Sinica, 2011, 27(1):255-261(in Chinese)
[20] Cao X, Shen F, Zhang M, et al. Efficient inner filter effect of gold nanoparticles on the fluorescence of CdS quantum dots for sensitive detection of melamine in raw milk[J]. Food Control, 2013, 34(1):221-229