静电纺丝SF/PLGA纤维支架用于血管组织工程的研究

摘要

将丝素蛋白（SF）和乳酸-羟基乙酸共聚物（PLGA）溶解在六氟异丙醇中配制成溶液，采用静电纺丝技术制备了SF/PLGA纳米纤维支架，使用扫描电子显微镜（SEM）对纤维支架进行表征，研究了聚合物溶液浓度、纺丝电压、接收距离以及体积流率对纳米纤维形态的影响，从而得到纺丝的最适宜工艺参数。考察了纤维支架表面对HUVECs细胞的相容性。结果表明：HUVECs可以在SF/PLGA纤维支架表面很好的黏附和增殖，支架具有良好的细胞相容性，在组织工程领域有良好的应用前景。

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	周伟
	冯亚凯

关键词：丝素蛋白； PLGA；静电纺丝；纤维支架；组织工程

Abstract：

Silk fibroin (SF) and lactic acid/glycolic acid copolymer (PLGA) were dissolved in 1,1,1,3,3,3-hexafluoroisopropanol to prepare SF/PLGA solution, and the electrospinning technique was used to prepare the SF/PLGA nanofibrous scaffolds. The scaffolds were characterized by SEM, and process influences on nanofiber morphology such as solution concentration, spinning voltage, receiving distance and volume flow rate were investigated in order to get the optimum process parameters of electrospinning. We tested the HUVECs cells compatibility of the nanofibrous scaffolds. The results showed that the SF/PLGA scaffolds could enhance the viability, spreading, and attachment of HUVECs, so these scaffolds have a good application prospect in the field of tissue engineering.

Keywords： silk fibroin； PLGA； electrospinning； fibrous scaffold； tissue engineering

Received 2014-05-09;

Fund:

国家自然科学基金（31370969），国家教育部博士点专项基金（20120032110073），科技部国际合作项目（2013DFG52040）。

Corresponding Authors: 冯亚凯,E-mail: yakaifeng@tju.edu.cn。 Email: yakaifeng@tju.edu.cn

About author: 周伟(1989-),男,硕士研究生,现从事静电纺丝方向的研究。

引用本文:

周伟, 冯亚凯.静电纺丝SF/PLGA纤维支架用于血管组织工程的研究[J]. 化学工业与工程, 2016,33(5): 56-61

Zhou Wei, Feng Yakai.Studies on Electrospun SF/PLGA Fibrous Scaffolds for Vascular Tissue Engineering[J]. Chemcial Industry and Engineering, 2016,33(5): 56-61

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