环糊精改性光子晶体分子印迹法检测诺氟沙星

摘要

首先合成顺丁烯二酸酐改性的β-环糊精,垂直沉积制备聚苯乙烯光子晶体模板。然后将改性环糊精与丙烯酸、过硫酸铵、N,N'-亚甲基双丙烯酰胺、诺氟沙星等混合的前驱液填入光子晶体模板中,引发交联聚合,得到蛋白石结构的凝胶光子晶体。再在二甲苯中超声除去聚苯乙烯光子晶体模板,用十二烷基硫酸钠的V(醋酸):V(甲醇)=1:9溶液除去分子印迹模板诺氟沙星,蒸馏水清洗后得到反蛋白石结构的分子印迹凝胶光子晶体。通过对比印迹凝胶光子晶体与非印迹凝胶光子晶体在同样条件下对诺氟沙星的响应性,发现印迹凝胶光子晶体能选择性地迅速响应溶液中的诺氟沙星,当诺氟沙星浓度从10^-9 mol/L递增到10^-4 mol/L时,其光子晶体的衍射峰位置从633 nm逐渐红移到722 nm,而非印迹凝胶光子晶体响应性很差。

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	张蓉
	刘晓燕
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关键词：光子晶体；分子印迹； β-环糊精；诺氟沙星

Abstract：

Maleic anhydride modified β-cyclodextrin (β-CD) was introduced into molecularly imprinting as a novel functional monomer and combined with photonic crystal to fabricate a molecularly imprinted photonic polymer (MIPP). Reactive β-CD monomer carrying vinyl carboxylic acid functional groups was firstly synthesized in our work, then the MIPP film with an opal structure was fabricated by filling precursor solution containing template (norfloxacin), functional monomers, crosslinker (N, N'-methylene bisacrylamide), initiator (ammonium persulfate) into the interstitial spaces of polystyrene photonic crystal templates, followed by a thermal polymerization at 55℃. The three-dimensional, highly-ordered and interconnected macroporous array inverse MIPP was obtained after the templates were removed by ultrasounded in xylene. The MIPP film can response norfloxacin within one minute, when the concentration of norfloxacin increased from 10^-9 mol/L to 10^-4 mol/L, the diffraction wavelength of the photonic crystal shifted from 633 nm to 722 nm. However, the NIPP film did not exhibit significant response.

Keywords： photonic crystal； molecular imprinting； β-cyclodextrin； norfloxacin

Received 2014-04-08;

Fund:

国家自然科学基金(21245006)。

Corresponding Authors: 余莉萍,E-mail:lipingyu@tju.edu.cn。 Email: lipingyu@tju.edu.cn

About author: 张蓉(1989-),女,硕士研究生,主要研究方向:光子晶体在分析中的应用。

引用本文:

张蓉, 刘晓燕, 余莉萍.环糊精改性光子晶体分子印迹法检测诺氟沙星[J]. 化学工业与工程, 2016,33(3): 39-44

Zhang Rong, Liu Xiaoyan, Yu Liping.Preparation of Cyclodextrin Modified Molecularly Imprinted Photonic Crystal for Detecting of Norfloxacin[J]. Chemcial Industry and Engineering, 2016,33(3): 39-44

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