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化学工业与工程 2012, Vol. 29 Issue (6) :69-73    DOI:
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红球菌H-412生长细胞脱除正十六烷中的有机硫
石立娜, 韩振为
1. 天津大学化工学院; 2. 天津大学精馏技术国家工程中心
Biodesulfurization of Organic Sulfur in n-Hexadecane by Rhodococcus  sp. H-412 Growing Cells
 SHI  Li-Na, HAN  Zhen-Wei
1. School of Chemical Engineering and Technology, Tianjin University;2. National Engineering and Research Center of Distillation Technology

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摘要 利用实验室筛选的能够通过“4S”途径进行脱硫代谢的红平红球菌H-412(Rhodococcus sp. H-412)作为生物脱硫催化剂,二苯并噻吩(DBT)为含硫模型化合物,考察了红球菌H-412生长细胞脱除正十六烷中有机硫的脱硫性能。在油相体积分数20%的反应体系中,相同浓度的生长细胞比休止细胞的脱硫效率高。利用红球菌H-412生长细胞在油相分率20%的情况下,初始发酵液菌体浓度为0.048 g/L,初始DBT浓度为0.25 mmol/L的条件下分批培养的脱硫效率较高,达到了57%。红球菌H-412 生长细胞在油相体积分率分别为0,5%,10%和20%条件下进行脱硫,DBT的消耗量及二羟基联苯(2-HBP)的生成量随油相体积增加而增大,20%条件下均达最大值,脱硫效率最高。细胞生长量随油相体积增大而减少,无油存在的条件下,细胞生长量最大。
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石立娜
韩振为
关键词生物脱硫   红球菌   生长细胞   二苯并噻吩     
Abstract: The Rhodococcus sp. H-412 screened in our lab, which could desulfurize via 4S pathway, was selected as biocatalyst to study the desulfurization performance by its growing cells, dibenzothiophene (DBT) and n-hexadecane were used for model organic-sulfur compound and model oil, respectively. The desulfurization efficiency (XDBS) of growing cells is higher than that of resting cells in the same biomass concentration when the oil phase was 20% (v/v). When initial biomass concentration was 0.048 g/L and DBT concentration was 0.25 mmol/L, the higher XDBS  was reached 57% under 20% (v/v) of hexadecane. The desulfurization by growing cells was carried out under the condition of 0%, 5%, 10%, 20% (v/v) of hexadecane, DBT consumption and 2-HBP formation increased with the increasing of oil phase, and the highest XDBS was reached when the hexadecane was 20%. Biomass growth reduced with the increasing of oil volume, under oil-free condition, the biomass reached highest value.
Keywordsbiodesulfurization   Rhodococcus sp.   growing cells   dibenzothiophene     
Received 2012-03-14;
Corresponding Authors: 韩振为,电话:(022)27404709,E-mail:hanzhenwei@tju.edu.cn。     Email: hanzhenwei@tju.edu.cn
About author: 石立娜(1987-),女,河北保定人,主要研究方向为微生物脱硫。
引用本文:   
石立娜, 韩振为.红球菌H-412生长细胞脱除正十六烷中的有机硫[J].  化学工业与工程, 2012,29(6): 69-73
SHI Li-Na, HAN Zhen-Wei.Biodesulfurization of Organic Sulfur in n-Hexadecane by Rhodococcus  sp. H-412 Growing Cells[J].  Chemcial Industry and Engineering, 2012,29(6): 69-73
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