壳聚糖絮凝法精制黄精水提取液

摘要采用单因素试验和正交试验，以黄精多糖保留率和除杂率为指标，优选壳聚糖絮凝法对黄精提取液进行精制的工艺。在单因素试验基础上选取药液质量体积比、壳聚糖加入量、絮凝pH值和絮凝温度4个因素进行正交试验。结果表明，壳聚糖絮凝法的最适宜工艺为：m(黄精):V(水提液)为4∶10（g∶mL），絮凝剂用量为药液体积的15%，絮凝的pH值为4.0，絮凝温度40 ℃，絮凝3 h。壳聚糖絮凝法对黄精多糖的除杂精制比普通醇沉法的效果明显，除杂率分别为16.08%和49.75%；多糖保留率分别为88.42%和60.34%，综合评分分别为66.72%和57.16%。

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	於娜
	黄山

关键词：黄精多糖壳聚糖絮凝法

Abstract： Based on the single factor experiment and the orthogonal design, taking the retention rate of polygonapolysaccharose and impurity removal rate as markers, the flocculation process by chitosan in treating the extracted polygonatum decoction was investigated. Experiment factors and levels were firstly selected by one factor tests. The four factors, concentration of waterextraction, chitosan dosage, pH value and flocculating temperature were chosen for the orthogonal design. The results showed the optimal conditions were: the concentration of waterextraction was 4∶10（g:mL）, the concentration of chitosan was 15% , the pH value was 4.0 , the flocculating temperature was 40 ℃ and the time was 3 h. Compared with alcohol precipitation method, by chitosan flocculating method, the flocculation rate was 16.08% and 49.75%; the retention rate of polygonapolysaccharose was 88.42% and 60.34%; colligation score was 66.72% and 57.16%, respectively. The flocculation of polygonatum L. extracted with chitosan is effective and it has greater advantage than the ethanol precipitation method.

Keywords： polygonatum polygona polysaccharose chitosan flocculating method

Received 2011-10-17;

Corresponding Authors: 黄山 Email: huangshan1975@163.com

About author: 於娜（1986-），女，河北唐山人，研究生，主要研究方向为新药及中药新剂型。

引用本文:

於娜, 黄山.壳聚糖絮凝法精制黄精水提取液[J]. 化学工业与工程, 2012,29(2): 32-36

YU Na, HUANG Shan.Flocculation of Decoction of Polygonatum with Chitosan[J]. Chemcial Industry and Engineering, 2012,29(2): 32-36